Resveratrol alleviates oxidative injury of lung cells caused by microparticles. (Average diameter < 2.5 micro...

Resveratrol alleviates oxidative injury of lung cells caused by microparticles. (average diameter < 2.5 μm) through reduced autophagy activity.

Abstract

Oxidative stress and inflammation are strongly associated with lung injury caused by airborne fine particles. (average diameter < 2.5 μm; PM2.5) Autophagy, which plays an important role in various physiological conditions. Helps adjust balance within cells and adapt to stress. Resveratrol is a phytoalexin that has powerful antioxidant effects against heart and lung disease. until now The mechanism by which resveratrol protects against PM2.5 remains to be elucidated. In this study We studied the effect of resveratrol on PM2.5-induced oxidative injury, the possible role of nuclear factor erythrod-2. The involvement of factor 2 and autophagy in this advance has been explored. Human bronchial lining cells were treated with PM2.5 and cytotoxicity and oxidative stress markers were determined. The study found that PM2.5 decreased cell viability and increased lactate dehydrogenase levels. Levels of malondialdehyde and reactive oxygen species were increased by exposure to PM2.5. PM2.5 also induced a significant increase in pro-inflammatory cytokines, including interleukins IL-6, IL-8, IL-1β, and tumor necrosis factor. Meanwhile, PM2.5 induced autophagy and alteration of nuclear factor erythrod-2 pathway. related to factor 2. Additionally, human bronchial epithelial cells were co-treated with PM2.5 and resveratrol in the presence or absence of 3-methylamphetamine which is an inhibitor of autophagy formation It was found that intervention with resveratrol partially eliminated PM2.5-induced oxidative injury through inhibiting autophagy regulation. The results of this study can provide new insights. On the molecular mechanisms of lung interference during exposure to PM2.5.