Effects of “Resveratrol” on human gastric cancer cells and try to explain the molecular mechanisms involved...

Summary of the article results of “Resveratrol” on human gastric cancer cells and attempt to explain the molecular mechanisms involved. Emphasis is placed on the MALAT1/miR-383-5p/DDIT4 signal pathway.

Summary of important points

Research objectives

Researchers wanted to determine whether resveratrol could:

☆ Inhibits the growth of stomach cancer cells.

☆ Reduce spread movement and invasion of cancer cells

☆ Stimulates the death of cancer cells (apoptosis).

Is it possible? And through what mechanism?

Method of study

The human gastric cancer cell line SGC7901 was administered to various concentrations of resveratrol (0, 1 and 5 µM) for 24 hours, then examined:

- increasing cell number

- Cell motility and invasion

- cell death

- Expression of genes and related microRNAs

Main research results

1. Resveratrol inhibits gastric cancer cells.

Resveratrol has been found to:

■ Reduce the proliferation of cancer cells.

■ Reduce migration and invasion.

■ Significantly increases apoptosis of cancer cells.

2. Reduce the activity of MALAT1

MALAT1 is a long non-coding RNA involved in the severity and spread of cancer.

Research has found that: Resveratrol decreases MALAT1 levels.

When MALAT1 activity was further suppressed, the anticancer effect became even more evident.

3. MALAT1 regulates miR-383-5p.

Researchers found: MALAT1 represses the activity of miR-383-5p.

When MALAT1 is decreased → miR-383-5p is increased.

4. miR-383-5p inhibits DDIT4.

DDIT4 is a protein involved in the survival and stress response of cancer cells.

The mechanism discovered is:

MALAT1 ↓ → miR-383-5p ↑ → DDIT4 ↓ → cancer cells were inhibited.

Summary of simple mechanisms

● Resveratrol → Decrease MALAT1 → Increase miR-383-5p → Decrease DDIT4 →

Causes cancer cells:

● Grows more slowly.

● Spread less.

● More cancer cells die.

The importance of research

This work supports that resveratrol has potential as an anti-gastric cancer agent. It works through regulating genes and microRNAs involved in cancer metastasis.